5LG6
Structure of the deglycosylated porcine aminopeptidase N ectodomain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.465, 215.690, 78.630 |
Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
Resolution | 24.982 - 2.500 |
R-factor | 0.1918 |
Rwork | 0.191 |
R-free | 0.22940 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.266 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | |
Number of reflections | 73869 | |
<I/σ(I)> | 6 | |
Completeness [%] | 97.0 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 5% PEG-1000, 10% PEG-8000 |