5LG1
Room temperature structure of human IgG4-Fc from crystals analysed in situ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.294, 81.934, 103.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.349 - 2.700 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c54 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.540 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.171 | |
| Number of reflections | 17764 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.7 | 8.9 |
| CC(1/2) | 0.992 | 0.433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES, 18-20% PEG 20 000 |
