5LG1

Room temperature structure of human IgG4-Fc from crystals analysed in situ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	291
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	73.294, 81.934, 103.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.349 - 2.700
R-factor	0.1781
Rwork	0.175
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c54
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.171
Number of reflections	17764
<I/σ(I)>	12.2	2
Completeness [%]	99.9	99.9
Redundancy	8.7	8.9
CC(1/2)	0.992	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES, 18-20% PEG 20 000