5LF9
Crystal structure of human NUDT22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.810, 52.114, 101.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.450 |
| R-factor | 0.17283 |
| Rwork | 0.171 |
| R-free | 0.20705 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.084 | 1.005 |
| Number of reflections | 47830 | |
| <I/σ(I)> | 15 | 2.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 15% HighPEG, 0.1 M hepes pH 7.5, 0.1 M NaKPO, 10% EDO |
