5LEM

Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	69.430, 169.950, 95.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.785 - 2.980
R-factor	0.2472
Rwork	0.245
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.434
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.785
High resolution limit [Å]	2.980
Number of reflections	23885
<I/σ(I)>	9.4
Completeness [%]	98.7
Redundancy	6.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	PEG6000 20% w/v, TAPS 0.02 M, pH 9.0