5LEB

Crystal structure of DARPin-DARPin rigid fusion, variant DDD_D12_06_D12_06_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	129.120, 29.470, 146.870
Unit cell angles	90.00, 107.47, 90.00

Refinement procedure

Resolution	35.343 - 2.300
R-factor	0.2127
Rwork	0.211
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2398))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.343	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	1.931
Number of reflections	24249
<I/σ(I)>	18.17
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 8000 10.0% w/v, PEG 1000 10.0% w/v, Sodium acetate 0.3 M, Sodium cacodylate 0.1 M, pH 6.5