5LE5

Native human 20S proteasome at 1.8 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	113.440, 202.770, 316.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	170.660 - 1.800
R-factor	0.18358
Rwork	0.182
R-free	0.21175
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3une
RMSD bond length	0.016
RMSD bond angle	1.778
Data reduction software	XDS
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	170.660	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.091	2.910
Number of reflections	633728
<I/σ(I)>	18.5	0.91
Completeness [%]	99.9	99.5
Redundancy	13.4	11
CC(1/2)	1.000	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris, 0.2 M Magnesium Chloride, 10 % PEG3350