5LE0

MICAL1 Cterminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978870
Spacegroup name	P 43 2 2
Unit cell lengths	52.780, 52.780, 157.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 3.300
R-factor	0.2725
Rwork	0.271
R-free	0.30630
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.597
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.043	0.317
Number of reflections	3734
<I/σ(I)>	26.56
Completeness [%]	99.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	EG