5LDA

Structure of deubiquitinating enzyme homolog (Pyrococcus furiosus JAMM1) in complex with ubiquitin-like SAMP2.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97939, 0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	47.110, 37.233, 59.528
Unit cell angles	90.00, 97.30, 90.00

Refinement procedure

Resolution	46.730 - 1.900
R-factor	0.176
Rwork	0.174
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.641
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.010
High resolution limit [Å]	1.900	1.900
Rmeas		0.557
Number of reflections	16059
<I/σ(I)>	14.84	1.99
Completeness [%]	98.0
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM Bis-Tris,200 mM NaCl, 25 % PEG3350, 10 % Glycerol