5LC8
Crystal Structure of specific mutant from Pseudomonas aeruginosa Lipoxygenase at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.332, 97.416, 155.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.710 - 1.800 |
| R-factor | 0.14102 |
| Rwork | 0.139 |
| R-free | 0.17079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ir4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.670 |
| Number of reflections | 59235 | |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 12 % PEG 3350, 0.2 M MgCl2, 0.1 M TRIS pH 7.1 |
