5LBN
High-resolution crystal structure of the UBC core domain of UBE2E1/UbcH6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.282416 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.301, 40.555, 91.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.060 - 1.420 |
| R-factor | 0.1558 |
| Rwork | 0.154 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.134 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.060 | 1.510 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.049 | 0.113 |
| Number of reflections | 26209 | |
| <I/σ(I)> | 33.21 | 13.15 |
| Completeness [%] | 94.8 | 79.2 |
| Redundancy | 11.568 | 9.66 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium citrate pH 6 and 8% (w/v) PEG 8000 |
