5LBI
Apo-structure of humanised RadA-mutant humRadA3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9799 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.350, 77.980, 39.810 |
Unit cell angles | 90.00, 117.53, 90.00 |
Refinement procedure
Resolution | 35.300 - 1.430 |
R-factor | 0.1652 |
Rwork | 0.164 |
R-free | 0.19280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a6p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.520 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.052 | 0.257 |
Number of reflections | 36018 | |
<I/σ(I)> | 20.51 | 5.56 |
Completeness [%] | 97.1 | 91.4 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 4-8% PEG1000, 100 mM Na/KPO4, pH 5.6 |