5LB5
Crystal structure of human RECQL5 helicase in complex with ADP/Mg (tricilinc form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.928190 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.590, 84.750, 106.691 |
| Unit cell angles | 108.82, 90.06, 96.86 |
Refinement procedure
| Resolution | 100.900 - 2.000 |
| R-factor | 0.2462 |
| Rwork | 0.244 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cdg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.900 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.114 | 1.510 |
| Number of reflections | 139875 | |
| <I/σ(I)> | 12.4 | 1.5 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M tris pH 7.5, 23% PEG3350, 0.1M sodium chloride |
