5LAA
X-RAY STRUCTURE OF THE METHYLTRANSFERASE SUBUNIT A FROM METHANOTHERMUS FERVIDUS IN COMPLEX WITH COBALAMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.602 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 100.604, 100.604, 262.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 131.350 - 3.000 |
| R-factor | 0.21057 |
| Rwork | 0.208 |
| R-free | 0.25424 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.302 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.350 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.224 | 1.060 |
| Number of reflections | 13764 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 98.5 | 91 |
| Redundancy | 17.1 | 11.9 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | Protein concentration 20 mg/mL complexed with hydroxy-cobalamin. A pink brick shape crystal appeared in 26% PEG 3000, 100 mM Na citrate pH 5.5. |
