5L9N
Structure of uridylylated GlnB from Escherichia coli bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97917 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 88.868, 88.868, 88.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.103 - 1.901 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pil |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.618 |
| Number of reflections | 9346 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0,1 M NaAcetate pH 4.6, 20 mM CaCl2, 30% MPD |
