5L9A
L-threonine dehydrogenase from trypanosoma brucei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.985, 57.722, 70.074 |
| Unit cell angles | 72.46, 70.38, 73.20 |
Refinement procedure
| Resolution | 46.820 - 1.450 |
| R-factor | 0.10254 |
| Rwork | 0.100 |
| R-free | 0.15643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yy7 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.820 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.089 | 0.398 |
| Number of reflections | 109054 | |
| <I/σ(I)> | 10.4 | 2.7 |
| Completeness [%] | 95.0 | 82.1 |
| Redundancy | 3.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 20 % w/v PEG 10K, TDH 2.0 mg/ml |
