5L8Z
Structure of thermostable DNA-binding HU protein from micoplasma Spiroplasma melliferum
Replaces: 5CVXReplaces: 4N1VExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.984 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.000, 39.010, 38.780 |
Unit cell angles | 90.00, 108.36, 90.00 |
Refinement procedure
Resolution | 31.640 - 1.400 |
R-factor | 0.1815 |
Rwork | 0.180 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1huu |
RMSD bond length | 0.019 |
RMSD bond angle | 2.229 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.26) |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.640 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.049 | 0.279 |
Rmeas | 0.058 | |
Rpim | 0.030 | |
Total number of observations | 54665 | |
Number of reflections | 15292 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 95.4 | 96.8 |
Redundancy | 3.6 | 3.5 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M TRIS, 35% PEG400, 5% glycerol |