5L8Z

Structure of thermostable DNA-binding HU protein from micoplasma Spiroplasma melliferum

Replaces:  5CVXReplaces:  4N1V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	KURCHATOV SNC BEAMLINE K4.4
Synchrotron site	KURCHATOV SNC
Beamline	K4.4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-22
Detector	MAR CCD 165 mm
Wavelength(s)	0.984
Spacegroup name	C 1 2 1
Unit cell lengths	57.000, 39.010, 38.780
Unit cell angles	90.00, 108.36, 90.00

Refinement procedure

Resolution	31.640 - 1.400
R-factor	0.1815
Rwork	0.180
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1huu
RMSD bond length	0.019
RMSD bond angle	2.229
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.26)
Phasing software	BALBES
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.640	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.049	0.279
Rmeas	0.058
Rpim	0.030
Total number of observations	54665
Number of reflections	15292
<I/σ(I)>	13.5
Completeness [%]	95.4	96.8
Redundancy	3.6	3.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1M TRIS, 35% PEG400, 5% glycerol