5L8V
Apo-structure of humanised RadA-mutant humRadA4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.610, 71.610, 74.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.420 - 1.500 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.093 | 0.544 |
| Number of reflections | 32967 | |
| <I/σ(I)> | 20.4 | 3.25 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 12% PEG20K, 0.1 M MES pH 6.5 |
