5L8N
crystal structure of human FABP6 protein with fragment 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.250, 64.430, 59.020 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.120 |
| R-factor | 0.24945 |
| Rwork | 0.247 |
| R-free | 0.30521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l8i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.059 | 0.738 |
| Number of reflections | 19956 | |
| <I/σ(I)> | 10.3 | 1.6 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 39% w/v PEG 3350 and 0.1 M Bis-tris pH 5.5 |
