5L8B
Crystal structure of Rhodospirillum rubrum Rru_A0973 mutant E62A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7291 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.090, 120.228, 140.358 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 48.870 - 2.210 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5da5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.098 | 1.009 |
| Number of reflections | 133316 | |
| <I/σ(I)> | 13.74 | 1.8 |
| Completeness [%] | 100.0 | 97 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1-2 ul protein mixed with 1 ul well solution. 15% PEG3350 + 0.13 M CaAc |
