5L88

AFAMIN ANTIBODY FRAGMENT, N14 FAB, L1- GLYCOSILATED, CRYSTAL FORM I, non-parsimonious model

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	67.782, 69.249, 87.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 1.880
R-factor	0.17212
Rwork	0.170
R-free	0.21145
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1il1
RMSD bond length	0.009
RMSD bond angle	1.377
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	BALBES
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.370	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.076	0.800
Number of reflections	34249
<I/σ(I)>	10	1.4
Completeness [%]	99.4	97.7
Redundancy	4.5	3.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	291	200nL Fab 10mg/ml in 20 mM HEPES pH 7.5, 150 mM NaCl plus 200nL 30%w/v PEG 1k, 200mM KF, pH 5.8)