5L7L

Crystal Structure of Elp3 from Dehalococcoides mccartyi (390-407 GSGSG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033200
Spacegroup name	C 2 2 21
Unit cell lengths	73.760, 161.410, 93.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.610 - 2.593
R-factor	0.1836
Rwork	0.182
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	trunctaed 5L7J
RMSD bond length	0.004
RMSD bond angle	0.684
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.590	2.590
Rmerge	0.126	2.974
Number of reflections	17592
<I/σ(I)>	14.8	0.94
Completeness [%]	99.6	94.9
Redundancy	13.06	11.8
CC(1/2)	0.999	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM MES pH 6.3 and 4% PEG 4000