5L7K
The crystal structure of myristoylated NPHP3 peptide in complex with UNC119a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97862 |
| Spacegroup name | I 41 |
| Unit cell lengths | 88.270, 88.270, 105.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.350 - 2.100 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rbq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.350 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.123 | 0.746 |
| Number of reflections | 22466 | |
| <I/σ(I)> | 13.47 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 1.75 M (NH4)2SO4, 0.1 M CAPS (pH 10.0) and 0.2 M Li2SO4 |
