5L78
Crystal structure of human aminoadipate semialdehyde synthase, saccharopine dehydrogenase domain (in NAD+ bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.222, 86.633, 149.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.010 - 2.680 |
| R-factor | 0.1902 |
| Rwork | 0.186 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l76 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.083 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.010 | 2.810 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.770 | |
| Number of reflections | 26685 | |
| <I/σ(I)> | 10.4 | 2.5 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M sodium chloride -- 25% PEG3350 -- 0.1M tris pH 8.5 |
