5L76

Crystal structure of human aminoadipate semialdehyde synthase, saccharopine dehydrogenase domain (in apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92819
Spacegroup name	P 43 21 2
Unit cell lengths	89.760, 89.760, 150.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.470 - 2.570
R-factor	0.2344
Rwork	0.232
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2axq
RMSD bond length	0.002
RMSD bond angle	0.489
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.440	2.640
High resolution limit [Å]	2.570	2.570
Number of reflections	20374
<I/σ(I)>	22.4
Completeness [%]	99.9	99.9
Redundancy	23.1	23.7
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350 -- 10% ethylene glycol -- 0.2M sodium malonate