5L74
Plexin A2 extracellular segment domains 4-5 (PSI2-IPT2), resolution 1.36 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2012-12-08 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.0163 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.710, 44.560, 33.010 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 52.092 - 1.360 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.298 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.100 |
| High resolution limit [Å] | 1.360 |
| Number of reflections | 27859 |
| <I/σ(I)> | 17 |
| Completeness [%] | 85.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.5 | 25% (w/v) polyethylene glycol 3,350 and 100 mM BIS-TRIS pH 5.5 |
