5L6L

Structure of Caulobacter crescentus VapBC1 bound to operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.972
Spacegroup name	C 2 2 21
Unit cell lengths	110.180, 144.160, 160.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.800 - 2.700
R-factor	0.1811
Rwork	0.178
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k8j
RMSD bond length	0.009
RMSD bond angle	1.108
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	67883
<I/σ(I)>	11.7	1.24
Completeness [%]	100.0	100
Redundancy	15.7	15.7
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292.15	20% PEG 3350, 0.2 Succinic acid pH 7, 5 mM MgCl2, 5 mM beta-mercapo ethanol, 20% glycerol