5L5N

Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8.5 angstrom, spacegroup P4(3)22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 43 2 2
Unit cell lengths	189.480, 189.480, 252.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.367 - 8.502
R-factor	0.4017
Rwork	0.401
R-free	0.41170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l56
RMSD bond length	0.019
RMSD bond angle	2.473
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.000
High resolution limit [Å]	8.500	8.500
Rmerge	0.330	1.170
Number of reflections	4374
<I/σ(I)>	4.2
Completeness [%]	99.7
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.5	1.5 lithium sulfate, 100 mM TRIS, pH 8.5