5L5L
Plexin A4 full extracellular region, domains 1 to 8 modeled, data to 8 angstrom, spacegroup P2(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.180, 241.000, 144.070 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 47.741 - 8.001 |
| R-factor | 0.3494 |
| Rwork | 0.349 |
| R-free | 0.34920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l56 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.464 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | |
| High resolution limit [Å] | 8.000 | 8.000 |
| Rmerge | 0.158 | 0.764 |
| Number of reflections | 10061 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.5 | 0.5 M sodium succinate, 100 mM bis-TRIS propane, pH 7.0 |
