5L5K

Plexin A4 full extracellular region, domains 1 to 10, data to 7.5 angstrom, spacegroup P4(1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 41
Unit cell lengths	220.593, 220.593, 65.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.148 - 7.501
R-factor	0.3537
Rwork	0.353
R-free	0.37140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l56
RMSD bond length	0.019
RMSD bond angle	2.439
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000
High resolution limit [Å]	7.500	7.500
Rmerge	0.207	1.210
Number of reflections	4253
<I/σ(I)>	12.2
Completeness [%]	99.8
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.5	29% (v/v) glycerol, 4.3% (w/v) PEG 8k, 53 mM TRIS hydrochloride, pH 8.5