5L5C
Plexin A1 full extracellular region, domains 1 to 10, to 6 angstrom, spacegroup P4(3)2(1)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 198.150, 198.150, 228.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.503 - 6.000 |
| R-factor | 0.3052 |
| Rwork | 0.305 |
| R-free | 0.31610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l56 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.242 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.503 | |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.710 | 1.240 |
| Number of reflections | 11641 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.5 | 6% (w/v) PEG 4k, 5 mM tricine, pH 8.5 |
