5L59
Plexin A1 full extracellular region, domains 1 to 10, to 6 angstrom, spacegroup P2(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0719 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 139.990, 196.450, 144.940 |
| Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
| Resolution | 58.218 - 6.000 |
| R-factor | 0.275 |
| Rwork | 0.274 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l56 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.250 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.218 |
| High resolution limit [Å] | 6.000 |
| Number of reflections | 19391 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 99.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.5 | 15% (v/v) propanol, 20 mM magnesium chloride 50 mM MES, pH 6.0 |
