5L4O

Structure of an E.coli initiator tRNAfMet A1-U72 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978570
Spacegroup name	C 2 2 21
Unit cell lengths	35.150, 142.620, 202.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.167 - 2.799
R-factor	0.1982
Rwork	0.197
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cw5
RMSD bond length	0.005
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.230	2.950
High resolution limit [Å]	2.790	2.790
Rmerge	0.084	2.464
Number of reflections	13192
<I/σ(I)>	17.03	0.95
Completeness [%]	99.2	95.5
Redundancy	9.84	9.74
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG 6000, 2M NaCl