5L3X

Crystal structure of negative elongation factor subcomplex NELF-AC

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-29
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	185.070, 185.070, 185.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.267 - 2.750
R-factor	0.2378
Rwork	0.237
R-free	0.25580
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.267	2.820
High resolution limit [Å]	2.750	2.750
Number of reflections	27492
<I/σ(I)>	32.2	1.9
Completeness [%]	100.0	100
Redundancy	39.8	40.7
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	14-14.5% (w/v) PEG 3350 200mM sodium malonate pH 6.8-7.0