5L3T

Structure of the Saccharomyces cerevisiae TREX-2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	78.170, 84.090, 165.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.081 - 4.927
R-factor	0.3395
Rwork	0.338
R-free	0.37010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t5g
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.081
High resolution limit [Å]	4.900
Number of reflections	5268
<I/σ(I)>	4
Completeness [%]	98.7
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Precipitant: 20% PEG 3350, 0.2 M Mg formate; protein 16.5 mg/ml in 0.3 M NaCl, 50 mM HEPES, pH 8, 5 mM DTT.