5L3Q
Structure of the GTPase heterodimer of human SRP54 and SRalpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 241.394, 241.394, 241.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.335 - 3.200 |
| R-factor | 0.1475 |
| Rwork | 0.145 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l3s |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.263 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.335 | 3.320 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 38582 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Tris pH 8.0, 400 mM lithium sulfate, 10 % (w/v) PEG 8000 |
