5L3F
LSD1-CoREST1 in complex with polymyxin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.204, 178.998, 235.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.227 - 3.500 |
| R-factor | 0.1868 |
| Rwork | 0.186 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.610 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.161 | |
| Number of reflections | 32150 | |
| <I/σ(I)> | 7.7 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.7 | 4.9 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.3 M Na/K Tartrate 100 mM ADA |
