5L38
The structure of the hexagonal shell protein MSM0272 from the RMM microcompartment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 |
| Unit cell lengths | 93.600, 93.600, 192.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.135 - 2.200 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.745 | |
| Number of reflections | 83627 | |
| <I/σ(I)> | 18.4 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.999 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Tris, 11% ethylene glycol, 10 % PEG 8000, 7mg/ml protein |
