5L32
Crystal structure of the Zn-RIDC1 complex bearing six interfacial disulfide bonds
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-16 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.060, 62.533, 72.437 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 37.810 - 2.100 |
| R-factor | 0.21563 |
| Rwork | 0.214 |
| R-free | 0.24972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hnj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.810 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.463 |
| Number of reflections | 24815 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 0.2 M magnesium chloride, 0.1 M Bis-Tris (pH 6.5), 35% PEG 400 |
