5L31
Crystal structure of an engineered metal-free RIDC1 variant containing five disulfide bonds.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.594, 77.534, 88.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.770 - 2.400 |
| R-factor | 0.22716 |
| Rwork | 0.225 |
| R-free | 0.26701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jea |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.302 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.230 | |
| Number of reflections | 17325 | |
| <I/σ(I)> | 14.9 | 5.7 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Drop consists of 1 uL of 45% MPD, and 0.1 M Bis Tris (pH 6.5) mixed with 1.5 uL of 2.8 mM protein |
