5L2W
The X-ray co-crystal structure of human CDK2/CyclinE and Dinaciclib.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2015-11-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.307, 100.307, 151.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.800 |
| R-factor | 0.2019 |
| Rwork | 0.200 |
| R-free | 0.24630 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1w98 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 19770 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 99.9 |
| Redundancy | 12.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286.15 | PEG20K, 180mM Mg(HCO2)2, 0.1M MES pH 6.0 |
