5L2U

Oligomer crystal structure of CC chemokine 5 (CCL5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-21
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97926
Spacegroup name	C 2 2 2
Unit cell lengths	121.799, 210.082, 126.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.648 - 2.280
R-factor	0.1793
Rwork	0.178
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cmd
RMSD bond length	0.008
RMSD bond angle	0.881
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.648
High resolution limit [Å]	2.280
Number of reflections	76849
<I/σ(I)>	33.74
Completeness [%]	99.8
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	303.15	0.1 HEPES, 300 mM NaCl, 3.5% 2-propanol