5L2Q

Serine/threonine-protein kinase 40 (STK40) kinase homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	55.550, 237.650, 55.610
Unit cell angles	90.00, 106.32, 90.00

Refinement procedure

Resolution	59.413 - 2.530
R-factor	0.2587
Rwork	0.258
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vz6
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.410	2.620
High resolution limit [Å]	2.530	2.530
Rmerge	0.060	0.780
Number of reflections	45250
<I/σ(I)>	12.52	1.49
Completeness [%]	98.6	99
Redundancy	3.4	3.5
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM Tris-HCL 7.5 21% PEG 4000 6% Glycerol 5% Tert-butanol 200mM NaCl 1mM TCEP