5L2H
Crystal Structure of W26A mutant of anti-EGFR Centyrin P54AR4-83v2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.350, 61.709, 59.515 |
| Unit cell angles | 90.00, 100.41, 90.00 |
Refinement procedure
| Resolution | 32.381 - 1.801 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.186 |
| Rmeas | 0.046 | |
| Rpim | 0.025 | |
| Total number of observations | 127861 | |
| Number of reflections | 38977 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.3 | 3 |
| CC(1/2) | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 25% PEG 3 kDa, 1 M sodium acetate, 0.1 M Tris pH 8.5 |
