5L23
Crystal structure of the complex between the N-terminal SH3 domain of CrkII and a proline-rich ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-05-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.600, 41.340, 56.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.430 - 1.770 |
| R-factor | 0.17587 |
| Rwork | 0.174 |
| R-free | 0.21037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cka |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.611 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.430 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.160 | 0.200 |
| Number of reflections | 6759 | |
| <I/σ(I)> | 11.7 | 8.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 0.977 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286 | Lithium sulfate monohydrate, PEG 3350 |
