5L1Z

TAR complex with HIV-1 Tat-AFF4-P-TEFb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1158
Spacegroup name	P 32 2 1
Unit cell lengths	146.870, 146.870, 103.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 - 5.900
Rwork	0.217
R-free	0.31320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 4OGR
RMSD bond length	0.003
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	500.000		5.410
High resolution limit [Å]	5.280	23.600	5.280
Rmerge	0.258	0.023
Rmeas	0.272	0.025
Total number of observations	48528
Number of reflections	4988	73	370
<I/σ(I)>	7.4	47.77	0.09
Completeness [%]	99.9	100	100
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	crystallized from 50 mM Tris 8.5, 0.2M AmmAcetate, 6 mM MgCl2, 8% PEG 4K at 291 degree K