5L1J
Crystal Structure of Human DNA Polymerase Eta Inserting dTMPNPP Opposite O6-Methyl-2'-deoxyguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07810 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.004, 99.004, 81.704 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.940 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.20166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o3n |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.091 | 0.819 |
| Number of reflections | 33771 | |
| <I/σ(I)> | 27.375 | 2.548 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M MES pH 5.5, 5mM Magnesium chloride, 16% PEG 2000 MME |
