5L1B
AMPA subtype ionotropic glutamate receptor GluA2 in Apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.051, 309.391, 110.021 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 48.550 - 4.000 |
| R-factor | 0.266 |
| Rwork | 0.265 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kg2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.584 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.098 | 1.573 |
| Number of reflections | 50426 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 98.0 | 98.8 |
| Redundancy | 3.27 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 8-11% (w/v) PEG 8,000, 0.2 M magnesium acetate and 0.1 M sodium cacodylate (pH 6.3-6.7) |
