5L1A
Crystal structure of uncharacterized protein LPG2271 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.154, 44.657, 54.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.449 - 2.400 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.24640 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.515 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2386) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.097 | 0.068 | 0.196 |
| Total number of observations | 24051 | ||
| Number of reflections | 4158 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 98.1 | 98.4 | 80.9 |
| Redundancy | 5.8 | 5.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 0.1M Citric acid, 25% PEG3350 |
