5L0V

human POGLUT1 in complex with 2F-glucose modified EGF(+) and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	70.810, 74.890, 83.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.810 - 1.305
R-factor	0.1593
Rwork	0.159
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.305
Rmerge	0.042	0.805
Number of reflections	103515
<I/σ(I)>	19.57	2.07
Completeness [%]	96.0	100
Redundancy	6.5	6.4
CC(1/2)	0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% PEG5000 MME, 50 mM MES pH 6.5, 10mM CaCl2, 250mM NaCl, 5% MPD