5L0M

hLRH-1 DNA Binding Domain - 12bp Oct4 promoter complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 43
Unit cell lengths	40.931, 40.931, 105.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.943 - 2.197
R-factor	0.158
Rwork	0.153
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a66
RMSD bond length	0.016
RMSD bond angle	1.651
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.095
Number of reflections	8862
<I/σ(I)>	24.3
Completeness [%]	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M calcium acetate and 20% (w/v) PEG 3350